6K1F
Crystal structure of the L-fucose isomerase from Raoultella sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 113.987, 127.611, 257.298 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.500 |
| R-factor | 0.17935 |
| Rwork | 0.177 |
| R-free | 0.23337 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fui |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.394 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.115 | 0.357 |
| Rmeas | 0.130 | 0.420 |
| Rpim | 0.059 | 0.216 |
| Number of reflections | 127420 | 6229 |
| <I/σ(I)> | 7.65 | 1.59 |
| Completeness [%] | 96.4 | 95.4 |
| Redundancy | 4.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | HEPES, PEG10000 |
