6K18

Crystal structure of EXD2 exonuclease domain soaked in Mn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	46.217, 73.866, 131.683
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.609 - 2.303
R-factor	0.2135
Rwork	0.210
R-free	0.27130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6k17
RMSD bond length	0.003
RMSD bond angle	0.645
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.103	0.348
Number of reflections	20317	939
<I/σ(I)>	12.35
Completeness [%]	98.3
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 8000, 2-(N-morpholino)ethanesulfonic acid, Calcium acetate, Dithiothreitol, Manganese chloride