6K12
Babesia microti lactate dehydrogenase apo form (BmLDH)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97736 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 255.604, 147.677, 65.266 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 45.655 - 2.794 |
| R-factor | 0.2811 |
| Rwork | 0.280 |
| R-free | 0.30790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ZXC |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.031 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.886 | 2.850 |
| High resolution limit [Å] | 2.794 | 2.800 |
| Rmeas | 0.100 | 0.100 |
| Number of reflections | 59522 | 59822 |
| <I/σ(I)> | 2 | |
| Completeness [%] | 99.4 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 0.2 M sodium acetate trihydrate PH 7.0, 11 ~ 15% w/v PEG3350 |
