6K0P

Catalytic domain of GH87 alpha-1,3-glucanase D1045A in complex with nigerose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-31
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	132.525, 132.525, 76.147
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.756 - 1.424
R-factor	0.1563
Rwork	0.156
R-free	0.17040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6k0m
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.855	1.510
High resolution limit [Å]	1.420	1.420
Rmerge	0.123	1.254
Number of reflections	125008	38213
<I/σ(I)>	9.2
Completeness [%]	98.9
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.5 M (NH4)2SO4, 25% (v/v) glycerol