6K0N

Catalytic domain of GH87 alpha-1,3-glucanase in complex with nigerose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-29
Detector	RAYONIX MX-225
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	132.656, 132.656, 76.236
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.820 - 1.600
R-factor	0.1496
Rwork	0.148
R-free	0.17230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6k0m
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.820	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.079	0.905
Number of reflections	89788	4456
<I/σ(I)>	32.5
Completeness [%]	100.0
Redundancy	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.5 M (NH4)2SO4, 25% (v/v) glycerol