6K0M

Catalytic domain of GH87 alpha-1,3-glucanase from Paenibacillus glycanilyticus FH11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-09-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1
Spacegroup name	P 43 21 2
Unit cell lengths	132.602, 132.602, 76.131
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.116 - 1.600
R-factor	0.1431
Rwork	0.142
R-free	0.16260
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.116	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.103	0.723
Number of reflections	89574	4426
<I/σ(I)>	27.8
Completeness [%]	100.0
Redundancy	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION		293	1.5 M (NH4)2SO4, 25% (v/v) glycerol