6K0F
The crystal structure of cyclopenin-bound AsqJ quinary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 73.839, 120.943, 66.722 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.211 - 1.634 |
R-factor | 0.1766 |
Rwork | 0.176 |
R-free | 0.19540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5y7r |
RMSD bond length | 0.007 |
RMSD bond angle | 1.073 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.690 |
High resolution limit [Å] | 1.630 | 1.630 |
Rmeas | 0.540 | |
Rpim | 0.270 | |
Number of reflections | 37248 | 3627 |
<I/σ(I)> | 29.2 | |
Completeness [%] | 99.7 | |
Redundancy | 4 | |
CC(1/2) | 0.839 | 0.839 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | PEG 1000, imidazole, calcium acetate |