6K02

Crystal structure of ceNAP1 core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9789
Spacegroup name	C 2 2 21
Unit cell lengths	95.733, 126.747, 118.192
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.196 - 2.162
R-factor	0.2517
Rwork	0.250
R-free	0.27920
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.200	2.240
High resolution limit [Å]	2.160	2.160
Rmerge		0.845
Number of reflections	38052	38052
<I/σ(I)>	20
Completeness [%]	99.2
Redundancy	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M Zinc acetate dihydrate, 0.1M Sodium cacodylate tridydrate pH=6.5, 18% PEG 8000