6JZJ

Structure of FimA type-2 (FimA2) prepilin of the type V major fimbrium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	58.030, 85.950, 102.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.750 - 1.600
R-factor	0.179
Rwork	0.178
R-free	0.19490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4q98
RMSD bond length	0.005
RMSD bond angle	0.757
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.800	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.051	0.514
Rmeas	0.064	0.656
Rpim	0.039	0.403
Number of reflections	68085	3344
<I/σ(I)>	13.7
Completeness [%]	99.9	100
Redundancy	4.7	4.7
CC(1/2)	0.999	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M HEPES-NaOH pH 7.5, 2.2M ammonium sulfate, 1.5% PEG 400