6JZF

Structure of the intermembrane space region of PARC6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	44.128, 121.669, 130.206
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.449 - 2.534
R-factor	0.2206
Rwork	0.218
R-free	0.27050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5had
RMSD bond length	0.004
RMSD bond angle	0.879
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.450	2.625
High resolution limit [Å]	2.534	2.534
Number of reflections	14753	760
<I/σ(I)>	13.2
Completeness [%]	93.8
Redundancy	10.7
CC(1/2)	0.992	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	MES, Ammonium sulfate, PEG 8000