6JZB
Structural characterization of DnaJ from Streptococcus pneumonia presents a new tetramer of Hsp40 family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 47.584, 104.242, 234.622 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.830 - 2.754 |
| R-factor | 0.2506 |
| Rwork | 0.249 |
| R-free | 0.28250 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.449 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.830 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.083 | |
| Number of reflections | 15553 | 777 |
| <I/σ(I)> | 21.15 | 3.2 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 7.1 | 7.2 |
| CC(1/2) | 0.902 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Jeffamine M-600 |
