6JZ7
b-glucuronidase from Ruminococcus gnavus in complex with N1-substituted uronic isofagomine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2019-02-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.539, 102.397, 110.776 |
| Unit cell angles | 90.00, 130.78, 90.00 |
Refinement procedure
| Resolution | 29.560 - 1.450 |
| R-factor | 0.146 |
| Rwork | 0.146 |
| R-free | 0.15900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5z19 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.155 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.500 |
| High resolution limit [Å] | 1.446 | 1.450 |
| Rmerge | 0.076 | 0.595 |
| Number of reflections | 358160 | 53100 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 70% MPD, 0.1 M HEPES, pH 7.5, 0.2 M CaCl2 |
