6JZ5
b-glucuronidase from Ruminococcus gnavus in complex with D-glucuronic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2018-10-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.549, 103.178, 112.460 |
| Unit cell angles | 90.00, 130.99, 90.00 |
Refinement procedure
| Resolution | 29.687 - 1.580 |
| R-factor | 0.1543 |
| Rwork | 0.154 |
| R-free | 0.17040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5z19 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.071 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.640 |
| High resolution limit [Å] | 1.579 | 3.400 | 1.580 |
| Rmerge | 0.068 | 0.039 | 0.402 |
| Rmeas | 0.075 | 0.043 | 0.449 |
| Rpim | 0.032 | 0.019 | 0.197 |
| Number of reflections | 313298 | 19151 | 18814 |
| <I/σ(I)> | 11 | ||
| Completeness [%] | 99.6 | 98.8 | 98.9 |
| Redundancy | 5 | 4.7 | 4.8 |
| CC(1/2) | 0.997 | 0.902 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 70% MPD, 0.1 M HEPES, pH 7.5, 0.2 M CaCl2 |
