6JYO

GII.13/21 noroviruses recognize glycans with a terminal beta-galactose via an unconventional glycan binding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	113
Detector technology	PIXEL
Collection date	2018-07-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97917
Spacegroup name	P 21 21 2
Unit cell lengths	87.642, 105.667, 138.196
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.350 - 1.502
R-factor	0.1887
Rwork	0.188
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.702
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	203722
<I/σ(I)>	22.114
Completeness [%]	99.9
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	8 percent Tacsimate pH 8, 20% polyethylene glycol 3350