6JYK

Crystal Structure of C. crescentus free GapR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9786
Spacegroup name	P 31 2 1
Unit cell lengths	55.007, 55.007, 110.999
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.776 - 2.000
R-factor	0.204
Rwork	0.203
R-free	0.21180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6k2j
RMSD bond length	0.009
RMSD bond angle	0.891
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.800	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.073	0.540
Number of reflections	13439	1291
<I/σ(I)>	37.7
Completeness [%]	98.0
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.01M Manganese (II) chloride tetrahydrate, 0.1M Sodium citrate (PH 5.6), 2.5M 1,6-Hexanediol and 0.3% v/v Dimethyl sulfoxide