6JYJ
Crystal structure of FAM46B (TENT5B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.605, 76.120, 135.075 |
| Unit cell angles | 90.00, 96.51, 90.00 |
Refinement procedure
| Resolution | 47.132 - 2.693 |
| R-factor | 0.206061753777 |
| Rwork | 0.204 |
| R-free | 0.25182 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FAM46C |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.531 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.132 | 2.772 |
| High resolution limit [Å] | 2.690 | 2.693 |
| Number of reflections | 33266 | 3214 |
| <I/σ(I)> | 14.12 | |
| Completeness [%] | 99.2 | 97.6 |
| Redundancy | 3.41 | 3.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 283 | 2% tacsimate pH5.0, 0.15M sodium citrate tribasic dehydrate pH5.6, 10% PEG3350 |
