6JYG
Crystal Structure of L-threonine dehydrogenase from Phytophthora infestans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-06 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 171.207, 98.865, 152.515 |
| Unit cell angles | 90.00, 106.72, 90.00 |
Refinement procedure
| Resolution | 49.280 - 2.310 |
| Rwork | 0.238 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a1n |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.096 | 0.451 |
| Number of reflections | 107201 | 5137 |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 93.3 | |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 293 | 40 % PEG600 0.1 M Citrate buffer (pH 5.8) |
