6JY3

Monomeric Form of Bovine Heart Cytochrome c Oxidase in the Fully Oxidized State

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	50
Detector technology	CCD
Collection date	2011-07-27
Detector	RAYONIX MX225HE
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	149.529, 152.131, 174.083
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.850
R-factor	0.15326
Rwork	0.151
R-free	0.19162
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5b1a
RMSD bond length	0.022
RMSD bond angle	2.039
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	200.000	1.820
High resolution limit [Å]	1.800	1.800
Rpim	0.031	0.459
Number of reflections	361138	12029
<I/σ(I)>	29.3	2
Completeness [%]	99.3
Redundancy	7.4
CC(1/2)		0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		277	sodium phosphate, PEG 4000, DM