6JX3
Lasso peptide synthetase B1 complexed with the leader peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-16 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 48.960, 90.300, 58.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.150 - 1.700 |
| R-factor | 0.1836 |
| Rwork | 0.182 |
| R-free | 0.20670 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.898 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.150 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 27376 | 2009 |
| <I/σ(I)> | 12.9 | 2.8 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 15% (v/v) Ethanol, 100 mM MES/ Sodium hydroxide pH 6.0, 200 mM Zinc acetate |
