6JWL

Crystal structure of EGFR 696-1022 L858R in complex with AZD9291

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97934
Spacegroup name	I 2 3
Unit cell lengths	143.518, 143.518, 143.518
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.430 - 2.551
R-factor	0.2085
Rwork	0.207
R-free	0.24160
RMSD bond length	0.013
RMSD bond angle	1.426
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.550	2.550
Number of reflections	16171
<I/σ(I)>	21.7
Completeness [%]	99.9
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	0.1M HEPES pH 7.8, 40% PEG 400, 0.15M NaCl, 5mM TCEP