6JWJ

Npl4 in complex with Ufd1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	74.005, 82.546, 94.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.550 - 1.580
R-factor	0.179097620233
Rwork	0.178
R-free	0.20557
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jwh
RMSD bond length	0.006
RMSD bond angle	0.984
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.610
High resolution limit [Å]	1.580	1.580
Number of reflections	77414	2809
<I/σ(I)>	25.6	0.554
Completeness [%]	99.6	70.8
Redundancy	12.4	7.5
CC(1/2)		0.496

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	90mM BisTris-HCl (pH 7.5), 19% PEG 3350, 10mM ATP