6JWH

Yeast Npl4 zinc finger, MPN and CTD domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.28260
Spacegroup name	P 21 21 21
Unit cell lengths	75.971, 82.936, 92.123
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.865 - 1.720
R-factor	0.164296845314
Rwork	0.163
R-free	0.19250
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.198
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.750
High resolution limit [Å]	1.720	1.720
Number of reflections	62488	2842
<I/σ(I)>	27.8	2.06
Completeness [%]	96.6	92.8
Redundancy	15.9	8.1
CC(1/2)		0.583

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	4% Tacsimat (pH 6.0), 12% PEG 3350