6JVZ
RVD HA specifically contacts 5mC through van der Waals interactions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-20 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.786, 88.022, 89.349 |
| Unit cell angles | 90.00, 104.77, 90.00 |
Refinement procedure
| Resolution | 86.400 - 2.480 |
| R-factor | 0.2259 |
| Rwork | 0.223 |
| R-free | 0.27520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gjp |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.717 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.400 | 2.520 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmeas | 0.096 | 0.908 |
| Number of reflections | 39755 | |
| <I/σ(I)> | 2.03 | |
| Completeness [%] | 98.8 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 8-10% PEG3350 (w/v), 10% ethanol, and 0.1 M MES pH 6.7 |
