6JVV
Crystal structure of maleylpyruvate hydrolase from Sphingobium.sp SYK-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-31 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.089, 74.519, 67.967 |
| Unit cell angles | 90.00, 118.56, 90.00 |
Refinement procedure
| Resolution | 31.630 - 1.510 |
| R-factor | 0.1526 |
| Rwork | 0.151 |
| R-free | 0.17370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pfz |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.934 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
| Rmerge | 0.054 | 0.039 | 0.350 |
| Rmeas | 0.058 | 0.043 | 0.398 |
| Rpim | 0.023 | 0.017 | 0.185 |
| Total number of observations | 562926 | ||
| Number of reflections | 91240 | 4672 | 2865 |
| <I/σ(I)> | 13 | ||
| Completeness [%] | 97.4 | 97.1 | 61.1 |
| Redundancy | 6.2 | 6.4 | 4 |
| CC(1/2) | 0.998 | 0.807 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG3350, Lithium sulfate |
