6JV9

Crystal Structure of Human CRMP2 1-532, unmodified

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	80.088, 158.425, 88.000
Unit cell angles	90.00, 94.19, 90.00

Refinement procedure

Resolution	50.000 - 2.260
R-factor	0.17181
Rwork	0.171
R-free	0.19337
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mkv
RMSD bond length	0.014
RMSD bond angle	1.520
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.260	2.260
Rmerge	0.163	0.579
Rpim	0.106	0.375
Number of reflections	102278	5113
<I/σ(I)>	6.5	2.5
Completeness [%]	99.9	99.9
Redundancy	3.3	3.3
CC(1/2)	0.960	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	0.1 M MES (pH 6.0), 15% (v/v) PEG 400, 0.1 M calcium acetate