6JV5

Crystal structure of 5-methylcytosine containing decamer dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	180
Detector technology	CCD
Collection date	2019-01-16
Detector	MAR CCD 130 mm
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	31.606, 25.788, 34.215
Unit cell angles	90.00, 115.93, 90.00

Refinement procedure

Resolution	30.769 - 1.401
R-factor	0.1824
Rwork	0.180
R-free	0.21660
RMSD bond length	0.005
RMSD bond angle	0.933
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.450
High resolution limit [Å]	1.400	1.400
Number of reflections	4932
<I/σ(I)>	29.5
Completeness [%]	99.1
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.02M Magnesium chloride hexahydrate,0.04MSodium cacodylate trihydrate pH 5.5, 40% MPD,0.002M Hexammine cobalt(III) chloride