6JV3
Crystal structure of 5-hydoxylmethylcytosine containing decamer dsDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2019-03-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.907, 23.818, 85.556 |
| Unit cell angles | 90.00, 111.12, 90.00 |
Refinement procedure
| Resolution | 26.604 - 2.851 |
| R-factor | 0.2309 |
| Rwork | 0.228 |
| R-free | 0.28210 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.423 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 2611 | |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 0.08M Sodium chloride,0.012M Potassium chloride, 0.02M Magnesium chloride hexahydrate 0.04M Sodium cacodylate trihydrate pH 6.0 30% MPD, 0.012M Spermine tetrahydrochloride |
