6JUY

Crystal Structure of ArgZ, apo structure, an Arginine Dihydrolase from the Ornithine-Ammonia Cycle in Cyanobacteria

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979176
Spacegroup name	C 1 2 1
Unit cell lengths	189.865, 105.439, 165.905
Unit cell angles	90.00, 113.18, 90.00

Refinement procedure

Resolution	41.060 - 2.970
R-factor	0.2354
Rwork	0.234
R-free	0.27470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c2q 3mgj 6jv0
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MoRDa
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.740	48.740	3.050
High resolution limit [Å]	2.970	13.280	2.970
Rmerge	0.039	0.024	0.564
Rmeas	0.044	0.028	0.637
Rpim	0.020	0.013	0.292
Total number of observations	263617	2946	20540
Number of reflections	60551	704	4524
<I/σ(I)>	18.4	42.7	2
Completeness [%]	97.3	95.1	99
Redundancy	4.4	4.2	4.5
CC(1/2)	0.999	0.999	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.24 M ammonium formate, 17% (w/v) PEG 3350