6JUO
MsDpo4-DNA complex 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.100, 81.030, 210.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.177 - 2.160 |
| R-factor | 0.20301 |
| Rwork | 0.200 |
| R-free | 0.25513 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jul |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.187 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.177 | 2.280 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.105 | 0.549 |
| Rpim | 0.039 | 0.199 |
| Number of reflections | 50583 | 7237 |
| <I/σ(I)> | 12.4 | 4.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.4 | |
| CC(1/2) | 0.996 | 0.884 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 297 | PEG 2KMME 12%, 0.2mM NaCl, Bis Tris Propane 6.0 |
