6JUG
Crystal Structures of Endo-beta-1,4-xylanase II Complexed with Xylotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2018-11-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.379, 59.140, 69.851 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.446 - 1.190 |
| R-factor | 0.1343 |
| Rwork | 0.134 |
| R-free | 0.14410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dfc |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.375 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.450 | 1.233 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Number of reflections | 63593 | 5906 |
| <I/σ(I)> | 19.6 | |
| Completeness [%] | 97.9 | 91.91 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 291 | PEG 8000, 0.2M NaI,0.1M MES |
