6JUE
The complex of PDZ and PBM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 63 |
| Unit cell lengths | 57.454, 57.454, 52.313 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.757 - 1.549 |
| R-factor | 0.2115 |
| Rwork | 0.211 |
| R-free | 0.22260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2k1z |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.729 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.757 | 1.630 |
| High resolution limit [Å] | 1.549 | 1.550 |
| Rmerge | 0.089 | 0.503 |
| Number of reflections | 14300 | 2038 |
| <I/σ(I)> | 14.8 | 3 |
| Completeness [%] | 99.5 | 98 |
| Redundancy | 9 | 4.8 |
| CC(1/2) | 0.997 | 0.783 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 2.4M ammonium sulfate and 0.1M citrate (pH 4.0) |
