6JTP

Crystal structure of HLA-C08 in complex with a tumor mut9m peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2017-07-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	68.280, 75.920, 78.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.550 - 1.900
R-factor	0.2424
Rwork	0.240
R-free	0.28280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.909
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.550	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.041	0.117
Number of reflections	32450	3174
<I/σ(I)>	7.15	4.02
Completeness [%]	96.0	99
Redundancy	2	2
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.2M ammonium acetate, 0.1M HEPES (pH 7.5), 25% w/v polyethylene glycol 3350