6JSX

Structure of a flagellin protein, HpFlaG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9641
Spacegroup name	P 65 2 2
Unit cell lengths	58.411, 58.411, 231.921
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.206 - 2.700
R-factor	0.2317
Rwork	0.227
R-free	0.26990
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.229
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.800
High resolution limit [Å]	2.700	5.800	2.700
Rmerge	0.054	0.032	0.574
Rmeas	0.058	0.035	0.619
Rpim	0.020	0.012	0.224
Number of reflections	7055	818	614
<I/σ(I)>	14.8
Completeness [%]	98.6	97.8	89.8
Redundancy	8.2	6.7	6.4
CC(1/2)		1.000	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	28% (NH4)2SO4, 0.1M K/Na Tartrate