6JSJ

Structural analysis of a trimeric assembly of the mitochondrial dynamin-like GTPase Mgm1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9785
Spacegroup name	P 41 21 2
Unit cell lengths	152.940, 152.940, 236.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.170 - 3.200
R-factor	0.246
Rwork	0.243
R-free	0.30600
Structure solution method	SAD
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (v1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.320
High resolution limit [Å]	3.200	3.200
Number of reflections	46877	3404
<I/σ(I)>	18.07
Completeness [%]	99.9
Redundancy	18
CC(1/2)	0.999	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	100mM potassium iodide, 8% PEG3350, 250mM NaCl, 10mM Tris (pH 8.0), 2mM magnesium chloride