6JSD
Crystal structure of the domain-swapped dimer H434A variant of the C-terminal domain of HtaA from Corynebacterium glutamicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-23 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.900, 40.230, 87.020 |
| Unit cell angles | 90.00, 113.97, 90.00 |
Refinement procedure
| Resolution | 39.608 - 2.042 |
| R-factor | 0.2056 |
| Rwork | 0.203 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jsa |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.752 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.120 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.053 | 0.816 |
| Rmeas | 0.062 | 0.959 |
| Rpim | 0.032 | 0.498 |
| Number of reflections | 21370 | 2022 |
| <I/σ(I)> | 15.06 | |
| Completeness [%] | 98.7 | 93.86 |
| Redundancy | 3.7 | 3.6 |
| CC(1/2) | 0.999 | 0.602 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG3350, Tris-HCl pH8, 2-propanol |
