6JS9

Crystal structure of the N-terminal domain of HtaA from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-13
Detector	RAYONIX MX300HE
Wavelength(s)	0.900
Spacegroup name	P 31 1 2
Unit cell lengths	65.120, 65.120, 181.980
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.409 - 2.000
R-factor	0.1962
Rwork	0.195
R-free	0.22000
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.984
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.410	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.096	0.619
Rmeas	0.103	0.667
Rpim	0.038	0.247
Number of reflections	30290	3011
<I/σ(I)>	11.01	3.03
Completeness [%]	99.9	100
Redundancy	7.1	7.1
CC(1/2)	0.997	0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	1.4 M tri-sodium citrate, 0.1 M HEPES NaOH pH7.0, 3% 2-methyl-2,4-pentanediol