6JRK

The structure of co-crystals of 8r-B-EGFR WT complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-08
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.97930
Spacegroup name	I 2 3
Unit cell lengths	146.542, 146.542, 146.542
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.341 - 2.796
R-factor	0.1999
Rwork	0.196
R-free	0.23560
RMSD bond length	0.009
RMSD bond angle	1.106
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.020
High resolution limit [Å]	2.800	2.800
Number of reflections	13045
<I/σ(I)>	24
Completeness [%]	100.0
Redundancy	19.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	38% PEG 300, 150 mM KCl, 0.1 M HEPES (pH 8.0), 50 mM Gly3, 5 mM Co-enzyme A (pH 8.0), 2 mM TCEP