6JRI

Crystal structure of Nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-24
Detector	MAR CCD 130 mm
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	70.305, 85.471, 229.145
Unit cell angles	90.00, 91.05, 90.00

Refinement procedure

Resolution	29.715 - 3.101
R-factor	0.2374
Rwork	0.235
R-free	0.25910
RMSD bond length	0.003
RMSD bond angle	0.792
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.715	3.212
High resolution limit [Å]	3.101	3.101
Number of reflections	25640
<I/σ(I)>	15.54
Completeness [%]	93.9
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		280	0.1 M sodium Acetate, 2.0 M sodium chloride