6JRE

Structure of N-terminal domain of Plasmodium vivax p43 (PfNTD) solved by Co-SAD phasing

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.59
Spacegroup name	P 41 21 2
Unit cell lengths	87.396, 87.396, 53.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.800 - 2.590
R-factor	0.2222
Rwork	0.220
R-free	0.27420
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.470
Data reduction software	DIALS
Phasing software	SOLVE
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.800	2.630
High resolution limit [Å]	2.590	2.590
Rmeas	0.081	3.353
Number of reflections	6539	241
<I/σ(I)>	27.1	1.2
Completeness [%]	95.8	71.8
Redundancy	58.1	28.3
CC(1/2)	1.000	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG 3350, 0.2M sodium bromide, 5% ethyl acetate