6JR6
Flavobacterium johnsoniae GH31 dextranase, FjDex31A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 102.471, 112.739, 114.250 |
| Unit cell angles | 111.13, 92.86, 114.54 |
Refinement procedure
| Resolution | 48.450 - 2.000 |
| R-factor | 0.1664 |
| Rwork | 0.165 |
| R-free | 0.19930 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.738 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.500 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.091 | 0.334 |
| Number of reflections | 275382 | 13574 |
| <I/σ(I)> | 7.8 | 1.8 |
| Completeness [%] | 97.1 | 96.1 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100 mM sodium acetate buffer, 8% (w/v) polyethylene glycol 20000, and 8% 2-methyl-2,4-pentanediol |
