6JR1

Crystal structure of the human nucleosome phased with 16 selenium atoms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-21
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	99.461, 108.539, 170.462
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.740 - 2.400
R-factor	0.2104
Rwork	0.209
R-free	0.24260
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.839
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.740	2.450
High resolution limit [Å]	2.400	2.400
Rmerge	0.133	0.989
Rmeas	0.138	1.029
Rpim	0.049	0.387
Number of reflections	72878	4431
<I/σ(I)>	15.7	2.9
Completeness [%]	100.0	100
Redundancy	14	13.3
CC(1/2)	0.999	0.845

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	potassium cacodylate, potassium chloride, manganese chloride