6JQZ
ZHD/H242A complex with ZEN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.680, 91.380, 113.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.090 |
| R-factor | 0.17226 |
| Rwork | 0.171 |
| R-free | 0.20343 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c8z |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.575 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 113.000 | 2.140 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.094 | 0.652 |
| Rpim | 0.056 | 0.387 |
| Number of reflections | 46691 | 3407 |
| <I/σ(I)> | 19.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.2 | |
| CC(1/2) | 0.998 | 0.925 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | ammonium phosphate, imidazole, potassium chloride |
