6JQK
Structure of C34M/N36
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97892 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 46.126, 46.126, 143.351 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.480 - 1.498 |
| R-factor | 0.2067 |
| Rwork | 0.205 |
| R-free | 0.22690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aik |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.803 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.480 | 1.590 |
| High resolution limit [Å] | 1.498 | 1.500 |
| Rmerge | 0.035 | 0.242 |
| Number of reflections | 9811 | 1561 |
| <I/σ(I)> | 20.34 | 3.43 |
| Completeness [%] | 98.2 | 94.3 |
| Redundancy | 3.96 | 2.89 |
| CC(1/2) | 0.999 | 0.967 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291.15 | 0.2M Sodium chloride,0.1 M BIS-TRIS pH 5.5,25% w/v PEG 3350 |
