6JQB

The structure of maltooligosaccharide-forming amylase from Pseudomonas saccharophila STB07 with pseudo-maltoheptaose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.978910
Spacegroup name	P 21 21 21
Unit cell lengths	47.900, 63.735, 163.727
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.292 - 1.101
R-factor	0.1568
Rwork	0.156
R-free	0.17070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iwk
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.120
High resolution limit [Å]	1.100	1.100
Rmerge	0.090	0.508
Number of reflections	211320	10184
<I/σ(I)>	1.36	5.432
Completeness [%]	99.8	99.2
Redundancy	12.6	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1 M ammonium sulfate, 0.2 M Tris-HCl, 20 mM acarbose, 20 mM maltoheptaose