6JQA

Crystal structure of phyllogen, a phyllody inducing effector protein of phytoplasma.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-27
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.7
Spacegroup name	P 21 21 21
Unit cell lengths	42.843, 60.664, 124.874
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.509 - 2.402
R-factor	0.2719
Rwork	0.269
R-free	0.30250
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.767
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX ((1.14_3260))
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.509	2.488
High resolution limit [Å]	2.402	2.402
Rmerge	0.137	0.949
Rmeas	0.147	1.013
Rpim	0.052	0.353
Number of reflections	194152	20164
<I/σ(I)>	8.21	1.91
Completeness [%]	85.6	52.53
Redundancy	7.9	8.2
CC(1/2)	0.996	0.713

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	100mM sodium citrate pH 5.0, 2.1M ammonium sulfate, 250mM MgCl2, 2.5% (v/v) glycerol