6JPK
Crystal structure of S. pombe aspartate aminotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.133, 53.111, 130.263 |
| Unit cell angles | 90.00, 96.70, 90.00 |
Refinement procedure
| Resolution | 39.294 - 2.102 |
| R-factor | 0.1443 |
| Rwork | 0.143 |
| R-free | 0.18270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yaa |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.817 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.075 | 0.143 |
| Rmeas | 0.083 | 0.160 |
| Rpim | 0.037 | 0.070 |
| Number of reflections | 49395 | 4880 |
| <I/σ(I)> | 31.4 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% (w/v) polyethyleneglycol 8000 0.1 M sodium HEPES |
