6JOY

The X-ray Crystallographic Structure of Branching Enzyme from Rhodothermus obamensis STB05

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.978910
Spacegroup name	P 43 21 2
Unit cell lengths	125.671, 125.671, 205.387
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.418 - 2.392
R-factor	0.2482
Rwork	0.247
R-free	0.27750
RMSD bond length	0.009
RMSD bond angle	1.114
Data reduction software	XDS
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.418	10.000
High resolution limit [Å]	2.390	2.390
Number of reflections	63390
<I/σ(I)>	2
Completeness [%]	96.7
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG 3350, HEPES, ammonia sulfate