6JNY

Crystal structure of bacteriophage 21 Q protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	32.902, 77.827, 68.692
Unit cell angles	90.00, 98.59, 90.00

Refinement procedure

Resolution	31.214 - 1.451
R-factor	0.2116
Rwork	0.211
R-free	0.22730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1or7
RMSD bond length	0.005
RMSD bond angle	0.735
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.480
High resolution limit [Å]	1.450	3.940	1.450
Rmerge	0.073	0.064	0.455
Rmeas	0.079	0.070	0.495
Rpim	0.030	0.027	0.190
Number of reflections	57774	2796	2581
<I/σ(I)>	18
Completeness [%]	95.5	89.9	85.4
Redundancy	6.9	6.7	6.5
CC(1/2)		0.995	0.929

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.2M KF and 14% PEG3350