6JNP
Structure of ExoT-SpcS Complex from Pseudomonas aeruginosa in 2.2 Angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER X8 PROTEUM |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-14 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.000, 63.000, 83.000 |
| Unit cell angles | 90.00, 98.00, 90.00 |
Refinement procedure
| Resolution | 34.793 - 2.261 |
| R-factor | 0.2145 |
| Rwork | 0.211 |
| R-free | 0.25350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jmf |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.525 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 23043 | 1072 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 90.3 | |
| Redundancy | 2.7 | |
| CC(1/2) | 0.991 | 0.957 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 295.1 | 25% PEG3350, 0.1 M Bis-Tris pH5.7-6.5 |
