6JMK

Ribosomal protein S7 from Mycobacterium tuberculosis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-10-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97928
Spacegroup name	P 21 21 21
Unit cell lengths	54.712, 58.428, 126.025
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.310 - 1.800
R-factor	0.18628
Rwork	0.185
R-free	0.21248
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hus
RMSD bond length	0.024
RMSD bond angle	2.257
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.310	1.864
High resolution limit [Å]	1.800	1.800
Rmerge	0.033	0.151
Rmeas	0.047	0.213
Number of reflections	38244	3755
<I/σ(I)>	11.88	4.09
Completeness [%]	100.0	100
Redundancy	2
CC(1/2)	0.996	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.2M potassium tartrate, 20% PEG 3350